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Transgenic crop plants have been created that do all of the following except


A) grow two kinds of crops, like the pomato which produces both tomatoes and potatoes.
B) are resistant to insect damage.
C) are resistant to herbicides.
D) produce human hormones or antibodies.
E) transgenic plants have been produced that can do all of the things listed.

F) A) and E)
G) B) and D)

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The study of proteomics is more complex than the study of genomics because


A) proteins are more complex than DNA.
B) proteins are harder to sequence than DNA.
C) there are 20 amino acids that comprise proteins and only 4 nucleotides that compose DNA.
D) scientists understand less about amino acids than they do about nucleotides.
E) each cell in an organism has exactly the same DNA but different cell types each produce different types of proteins.

F) C) and E)
G) A) and E)

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Ex vivo gene therapy


A) requires the removal of cells from the individual undergoing the treatment and then the reintroduction of these cells once they have been genetically modified.
B) requires the use of an agent to introduce genetic material directly into the body using a vector such as an adenovirus vector.
C) has few or no side effects on the individual receiving the treatment.
D) allows for a wider range of disorders to be treated than in vivo treatments.
E) is considered to be more dangerous for the patient than in vivo treatment.

F) B) and E)
G) A) and B)

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Which method would be the most realistic to use in the development of frost resistant crop plants?


A) Modify bacteria that live on plants that cause ice crystals to form.
B) Cause the plant's DNA to produce some of the chemicals found in antifreeze.
C) Mixing parts of the DNA of arctic plants into the DNA of the crop plant.
D) Use synthetic DNA that is based on the DNA of arctic mammals, like polar bears.
E) Use the DNA of a type of Archaea known to live in extremely cold areas.

F) A) and B)
G) B) and C)

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What is the substance required to cleave the vector DNA during recombinant DNA technology?


A) restriction enzymes
B) DNA ligase
C) plasmids
D) vectors
E) DNA helicase

F) A) and B)
G) A) and C)

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Which of the following are sites of in vivo gene therapy?


A) muscle cells
B) bone marrow
C) endothelium
D) liver
E) skin

F) A) and E)
G) B) and D)

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Gene therapy may be used in the future to fight cancer by inserting genes that


A) fight off mutations of the patient's DNA.
B) produce radioactive isotopes.
C) cause cell death.
D) produce anticancer drugs.
E) all of the above.

F) A) and B)
G) None of the above

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In producing transgenic plants,cells called protoplasts are often used.Protoplasts are plant cells from which the cell wall has been removed.Why would the cell wall need to be removed?


A) The foreign DNA enters the cell through self-healing holes in the membrane but it could not pass through the cell wall.
B) The cell wall prevents the transgenic cell from growing into a mature plant.
C) The cell wall makes it difficult for the biotechnician to determine if the foreign DNA has been incorporated into the cell's genome.
D) The cell wall prevents the microinjection of foreign DNA into the cell because the needle cannot pass through it.
E) The foreign DNA passes through the plasmodesmata of the plant cell which is blocked by the cell wall.

F) None of the above
G) B) and D)

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Which of the following is not part of the process for PCR (polymerase chain reaction) ?


A) The use of an engineered DNA polymerase.
B) The denaturing of the double stranded DNA to yield single strand DNA.
C) Multiple cycles of the chain reaction to yield a greater number of DNA copies.
D) The final product is single stranded DNA.
E) All of these are parts of the process for PCR.

F) A) and B)
G) C) and D)

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When creating transgenic animals as contrasted with the creation of transgenic bacteria


A) It is necessary to carry out the procedure completely by hand.
B) Animals need donor eggs to contain the genetic material while bacteria can have the new genes inserted into any bacterial cell.
C) Transgenic animals can be created in large numbers while bacteria must be created one at a time to control contamination.
D) The membranes of the bacteria must be disrupted to allow the genes to be taken up into the cell and animals do not require the host cell membrane to be disrupted.
E) The membranes of the animal cell must be disrupted to allow the genes to be taken up into the cell and bacteria do not require the host cell membrane to be disrupted.

F) All of the above
G) A) and B)

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Ex vivo gene therapy is being used to treat patients with Huntington disease,Alzheimer disease,Parkinson disease,and brain tumors.

A) True
B) False

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Which of the following is NOT correct regarding restriction enzymes?


A) They exist in bacteria to restrict the growth of viruses.
B) They cut double-stranded DNA at specific sites.
C) They are used during the manufacture of proteins.
D) They produce "sticky ends" that can bind foreign DNA.
E) They facilitate inserting foreign DNA into vector DNA.

F) B) and D)
G) A) and C)

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A piece of DNA that contains sequences from two or more different organisms is called ______ DNA.


A) inverse
B) species-specific
C) recombinant
D) transgenic
E) semi-conservative

F) A) and D)
G) All of the above

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The study of comparative genomics has given researchers the ability to look at multiple genomes from different species which has lead to new ideas about the evolutionary history of organisms.The conclusion is that


A) Modern vertebrate species all evolved from very different ancestral species so there is little similarity between their genomes.
B) Modern vertebrate species all evolved from the same ancestral species but they have changes so that their genomes are very different in modern times.
C) Ancestral species contained very similar genomes but modern vertebrate species have significant differences in their genomes.
D) Modern vertebrate species evolved from the same ancestors and a large portion of their genome is conserved.
E) Neither the ancestral species nor the modern one resemble each other genetically.

F) A) and B)
G) A) and C)

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List the steps required for insertion of foreign genes into animal eggs by using vortex mixing.

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1.The eggs are placed in an agitator wit...

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Transposons are transposable elements in the DNA because


A) they transport genetic information.
B) they transfer organelles inside a cell.
C) they transmit signals in the cell indicating the genes to be copied.
D) they move from one location to another in the DNA.
E) they cause the DNA to take on a specific shape or pose.

F) C) and D)
G) A) and B)

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In STR,short tandem repeat,profiling,both _______________ and _____________________ are used to identify an individual.


A) length or the repeated sequence: number of fragments
B) rDNA: tRNA
C) specific genetic sequences: types of mRNA
D) mitochondrial DNA: number of fragments: number of fragments
E) mitochondrial DNA: specific genetic sequences

F) All of the above
G) D) and E)

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Genetically modified organisms (GMOs) that are used as crop plants to feed humans are able to express new traits because


A) they now contain genes from another organism that code for these molecules.
B) they grow bacteria on the surface of leaves or roots that produce these molecules.
C) they have been injected with these new molecules and there is no natural method for them to be degraded in the organism.
D) their normally occurring molecules have been modified to serve different functions.
E) they are hybrid plants that contain new alleles.

F) None of the above
G) A) and C)

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Gene pharming is


A) the use of bacterial DNA to impart drug resistance to plants.
B) the use of transgenic farm animals to produce pharmaceuticals.
C) the creation of new genes to be implanted in farm animals.
D) the creation of crop plants to replace the use of farm animals as a source of protein.
E) the application of genetic principles to breeding farm animals.

F) C) and E)
G) B) and D)

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The two techniques typically used to create transgenic animals are


A) microinjection of eggs and vortex mixing of eggs.
B) microinjection of eggs and electroshock of eggs.
C) electroshock of protoplasts and electroshock of eggs.
D) microinjection of protoplasts and electroshock of eggs.
E) microinjection of eggs and vortex mixing of protoplasts.

F) B) and E)
G) A) and E)

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