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The type of microscope in which you would see brightly illuminated specimens against a black background is


A) bright-field.
B) dark-field.
C) phase-contrast.
D) fluorescence.
E) electron.

F) C) and E)
G) A) and E)

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The _____ of the microscope holds and allows selection of the objective lenses.

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All of the following are examples of different types of microbiological media except


A) broth.
B) enriched.
C) agar.
D) petri dish.
E) gelatin.

F) A) and D)
G) B) and E)

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How will


A) Gram-positive
B) Gram-negative
C) Gram-variable
D) None of these
E) coli appear if the mordant is not applied?

F) A) and E)
G) A) and D)

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A contaminated food sample contains several different species of bacteria.A food microbiologist is interested in studying just one of these species.Describe the sequence of procedures that the microbiologist must perform in order to obtain a pure culture of the bacterial species of interest from this food sample.Detail all the necessary media and equipment.

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The microbiologist must first obtain a s...

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The procedure for culturing a microorganism requires the use of a microscope.

A) True
B) False

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A media is designed that allows only staphylococci to grow.In addition, S.aureus colonies have a yellow halo around them and other staphylococci appear white.This type of media is


A) selective only.
B) differential only.
C) both selective and differential.
D) a reducing media.
E) enriched.

F) B) and C)
G) C) and D)

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All of the following are diameters of cells that would be resolved in a microscope with a limit of resolution of 0.2 µm except


A) 0.2 µm.
B) 0.2 mm.
C) 0.1 µm.
D) 0.3 µm.
E) 2.0 µm.

F) A) and D)
G) D) and E)

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The specimen preparation that is best for viewing cell motility is


A) hanging drop.
B) fixed stained smear.
C) gram stain.
D) negative stain.
E) flagellar stain.

F) B) and E)
G) A) and C)

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Explain the difference and significance between a contaminated culture and a mixed culture.

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A contaminated culture refers to a cultu...

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Which of the following will result when 1% to 5% agar is added to nutrient broth, boiled and cooled?


A) a pure culture
B) a mixed culture
C) a solid medium
D) a liquid medium
E) a contaminated medium

F) None of the above
G) C) and E)

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Which microscope bombards a whole, metal-coated specimen with electrons moving back and forth over it?


A) fluorescence
B) differential interference contrast
C) scanning electron
D) transmission electron
E) phase-contrast

F) A) and B)
G) B) and D)

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Brain-heart infusion, trypticase soy agar (TSA) , and nutrient agar are all examples of which type of media?


A) synthetic
B) reducing
C) enriched
D) nonsynthetic
E) selective

F) A) and C)
G) None of the above

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The primary purpose of staining cells on a microscope slide is to


A) kill them.
B) secure them to the slide.
C) enlarge the cells.
D) add contrast in order to see them better.
E) see motility.

F) C) and E)
G) A) and B)

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Basic dyes are


A) attracted to the acidic substances of bacterial cells.
B) anionic.
C) used in negative staining.
D) repelled by cells.
E) dyes such as India ink and nigrosin.

F) A) and B)
G) B) and E)

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_____ dyes have a negative charge on the chromophore and are repelled by bacterial cells.

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Which of the following reagents reacts with crystal violet as the mordant?


A) Crystal violet
B) Gram's iodine
C) 95% ethyl alcohol
D) Safranin

E) A) and D)
F) None of the above

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A microbiologist inoculates Staphylococcus epidermidis and Escherichia coli into a culture medium.Following incubation, only the E.coli grows in the culture.What is the most likely explanation?


A) The microbiologist used too much inoculum.
B) The culture is contaminated.
C) The incubation temperature was incorrect.
D) The culture medium must be selective.
E) The culture medium must be differential.

F) B) and D)
G) B) and E)

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A nutrient medium that has all of its chemical components identified and their precise concentrations known and reproducible would be termed


A) complex.
B) reducing.
C) enriched.
D) enumeration.
E) synthetic.

F) C) and D)
G) A) and D)

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Bacteria that require special growth factors and complex organic substances are called


A) fastidious.
B) pathogenic.
C) harmless.
D) anaerobic.
E) aerobic.

F) A) and B)
G) B) and D)

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