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Exam 17: Procedures for Identifying Pathogens and Diagnosing Infections

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Question 21

Multiple Choice

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In lab, a student observes some dull, wrinkled brown colonies growing on their agar.They gram stain the sample and observe single bacilli.Their blood agar does not reveal any hemolysis while their MSA has growth and fermentation.Using the following table and the given observations, determine which microorganism this would most likely be.

A) E. coli
B) Pseudomonas aeruginosa
C) B. subtilis
D) B. cereus

E) C) and D)
F) B) and C)

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Question 22

Multiple Choice

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Serotyping of the pneumococcus based on capsular polysaccharide is performed in:

A) Ouchterlony double diffusion.
B) Western blot.
C) immunelectrophoresis.
D) radioimmunoassay (RIA) .
E) the Quellung test.

F) B) and D)
G) None of the above

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Question 23

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A simple method to detect _____ cells involves mixing them with untreated sheep red blood cells that will bind and form rosettes.

Affixing antigen to an inert particle such as a latex bead is sometimes used in which type of test?

Identify, describe, and discuss the objectives of the three main categories of identifying infectious agents using specific examples.

In direct antigen testing, the reaction is generally seen with the:

The Widal test is used for diagnosing salmonellosis.

Which technique used to diagnose a disease is also called the double diffusion method?

Mary had an accidental needlestick one night during her shift as an emergency medical technician.Initially, she tested positive for HIV by the ELISA test.Which test would be done next to confirm an HIV infection?

Broad general characteristics of bacteria on flowcharts can be misleading for a diagnosis of disease.

Serological tests should have low sensitivity and specificity.

Which of the following choices are true about viral hemagglutination testing?

The more a sample can be diluted and yet still react with antigen, the ______ the concentration of antibodies in that sample and the _______ is its titer.

Which test is used to measure the IgE present in allergic patients?

Soluble antigens are detected in which type of test?

When collecting __________, a "clean catch" method is often used.

Serological testing always involves reactions between specific antibody and antigen.

The indirect ELISA test detects ______ in a patient's blood.

When antibodies or other substances in serum cross-react with the test reagents, a false positive result can occur.

Why do many physicians feel that stool cultures are not necessary, except in certain circumstances when diagnosing GI tract infections?