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How does the structure of the outer side of the nuclear pore complex differ from that of the inner side?

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On the outer side, eight cytoplasmic fil...

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The eukaryotic large ribosomal subunit contains the 28S, 5.8S, and 5S rRNAs, plus many different proteins.

A) True
B) False

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The activity of transcription factors is frequently regulated by controlling their localization within eukaryotic cells, such that only in response to a given stimulus is the transcription factor transported into the nucleus, where it can activate its target genes. For a transcription factor that has a nuclear localization signal (NLS), describe two mechanisms by which regulated transport can occur.

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Two possible mechanisms involve masking ...

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Nuclear localization signals are usually short sequences rich in which amino acids?

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In ribosome assembly, _______ that were synthesized in the cytoplasm are assembled into complexes on pre-ribosomal RNA molecules.

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proteins (...

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Individual chromosomes occupy distinct _______ within the nucleus.

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territorie...

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The genes encoding 5.8S, 18S, and 28S ribosome RNA are clustered together in


A) tandem arrays located on one chromosome.
B) tandem arrays interspersed with tandem arrays of genes encoding 5S ribosomal RNA.
C) tandem arrays located on three chromosomes.
D) tandem arrays located on five chromosomes.

E) None of the above
F) B) and D)

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By what mechanism are most nuclear proteins specifically targeted to the nucleus for import?

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They have a nuclear import signal (a sho...

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The nuclear envelope consists of what four major components?

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The inner nuclear membrane, th...

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Emery-Dreifuss muscular dystrophy and Hutchinson-Gilford progeria are caused by mutations in genes that code for


A) importin or Ran.
B) Polycomb proteins.
C) NF-κB.
D) A-type nuclear lamins or emerin.

E) A) and D)
F) A) and C)

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Small molecules (< 20 kd) enter nuclei by way of


A) passive diffusion through nuclear pore complexes.
B) selective transport through nuclear pore complexes.
C) active transport across the nuclear envelope membranes.
D) passive diffusion across the nuclear envelope membranes.

E) All of the above
F) A) and B)

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The nuclear localization signal is typically a(n) _______, rich in the amino acids _______.


A) α helix; Pro, Lys, and Arg
B) α helix; Leu, Phe, and Gly
C) short sequence; Lys and Arg
D) short sequence; Pro, Leu, and Gly

E) A) and D)
F) C) and D)

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The mass of the nuclear pore complex is estimated to be


A) smaller than that of a ribosome.
B) about the same as that of a ribosome.
C) about five times that of a ribosome.
D) about 30 times that of a ribosome.

E) B) and C)
F) A) and C)

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Receptor-dependent protein transport into the nucleus requires the recognition of a _______ on the cargo protein.

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nuclear lo...

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Entry into the nucleus is limited by the _______ and the _______ complexes.

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nuclear envelope (nu...

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Because of the presence of a nuclear envelope, eukaryotes can perform several functions that prokaryotes cannot. Identify one of these functions.

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Answers will vary, including p...

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What modification of nucleotides occurs during processing of pre-rRNA?

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Addition o...

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In eukaryotic cells, the contents of the nucleus are separated from the cytoplasm by the _______.

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nuclear en...

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Polycomb proteins consist of _______ complex(es) and are concentrated in Polycomb bodies within the nucleus.


A) 1
B) 2
C) 3
D) either 2 or 3, depending on cell type

E) A) and D)
F) B) and C)

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Cajal bodies are thought to represent sites of


A) DNA replication.
B) snRNA processing and assembly.
C) large ribosomal subunit assembly.
D) small ribosomal subunit assembly.

E) None of the above
F) All of the above

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