Question 1

Semiconservative replication refers to ________.

Accepted Answer

A)   each base bonding at the 1' position of the sugar
B)   a purine always bonding to a pyrimidine
C)   one helix strand that runs from the 5' to 3' direction and the other strand runs from the 3' to 5' direction
D)   an original parent DNA strand and one newly synthesized DNA strand comprising a new DNA molecule
E)   None of the choices are correct.F) A) and E)
G) A) and B)

Question 2

The nontranscribed region of DNA to which RNA polymerase binds to initiate transcription is called the ________.

Accepted Answer

A)   promoter
B)   operator
C)   operon
D)   exon
E)   intronF) A) and D)
G) All of the above

Question 3

The site where the old DNA strands separate and new DNA strands will be synthesized is called the ________.

Accepted Answer

A)   primer
B)   Okazaki fragment
C)   template
D)   rolling circle
E)   replication forkF) All of the above
G) D) and E)

Question 4

DNA is denatured at ________.

Accepted Answer

A)   37oC
B)   42oC
C)   60oC
D)   94oC
E)   100oCF) A) and D)
G) A) and C)

Question 5

A three base sequence on mRNA is called a(n)  ________.

Accepted Answer

A)   codon
B)   exon.
C)   anticodon
D)   intronE) C) and D)
F) B) and C)

Question 6

The RN educates the patient that genetic information can be acquired by bacterial cells in other ways. Which of the following is a correct statement regarding these processes?

Accepted Answer

A)   Transformation requires the formation of a pilus for genetic exchange to occur.
B)   Transposition involves the transfer of genetic information to bacterial cells directly from their environment.
C)   Genetic transfer of transposons involves a random fragment of DNA being taken up by a phage.
D)   Transduction involves the transfer of genetic information from one bacterial cell to another using a bacteriophage as the vector.E) B) and C)
F) None of the above

Question 7

Three consecutive bases in the DNA of a gene represent the code for one ________.

Accepted Answer

A)   protein
B)   nucleotide
C)   amino acid
D)   purine
E)   pyrimidineF) A) and E)
G) A) and C) C

Question 8

Restriction endonucleases recognize and clip at DNA base sequences called ________.

Accepted Answer

A)   codons
B)   palindromes
C)   introns
D)   exons
E)   genesF) A) and D)
G) A) and E)

Question 9

RNA molecules differ from DNA molecules because only RNA ________.

Accepted Answer

A)   has ribose
B)   has uracil
C)   is typically one strand of nucleotides
D)   does not have thymine
E)   All of the choices are correct.F) B) and D)
G) B) and C)

Question 10

The smallest unit of heredity is a chromosome.

Accepted Answer

A) True 
 B)False

Question 11

The benefit of the bacterial operon is that ________.

Accepted Answer

A)   since the proteins coded for by the operon structural genes work as a team, they are regulated (i.e. induced or repressed)  as a team, thus saving energy
B)   the structural genes can be regulated individually, affording the cell more control over the protein products
C)   the split genes can be cut and spliced back together with greater precision than with single genes
D)   they are much less prone to mutation than individual genesE) C) and D)
F) A) and C)

Question 12

Replication of DNA begins at a(n)  ________ rich area.

Accepted Answer

A)   guanine-cytosine.
B)   uracil-adenine.
C)   adenine-thymine.
D)   adenine-cytosine.
E)   guanine-adenine.F) B) and C)
G) A) and E)

Question 13

Vectors often contain a gene conferring drug resistance to their cloning host, in order to detect cells harboring the plasmid.

Accepted Answer

A) True 
 B)False

Question 14

Which is incorrect about purines?

Accepted Answer

A)   Only found in DNA, not in RNA
B)   Are nitrogenous bases
C)   Always paired with a specific pyrimidine
D)   Include adenine and guanine
E)   Found within nucleotidesF) None of the above
G) A) and B)

Question 15

DNA Polymerase I ________.

Accepted Answer

A)   adds bases to new DNA chain
B)   supercoils DNA
C)   removes primers
D)   unzips DNA
E)   synthesizes RNA primerF) None of the above
G) A) and D) C

Question 16

Which of the following is not associated with a bacterial ribosome?

Accepted Answer

A)   Is a 70S
B)   Has a peptide (P)  site
C)   Has an exit site
D)   Small unit provides the enzymes for making peptide bonds
E)   Has an amino acid (
A)   siteF) B) and E)
G) C) and D)

Question 17

DNA replication proceeds in one direction around the bacterial chromosome.

Accepted Answer

A) True 
 B)False

Question 18

DNA polymerase III ________.

Accepted Answer

A)   is needed for adding nucleotides during mRNA synthesis
B)   synthesizes new DNA only in the 5' to 3' direction
C)   cannot add nucleotides to the lagging strand
D)   synthesizes an RNA primer
E)   All of the choices are correct.F) A) and E)
G) B) and D) B

Question 19

The enzymes that can proofread replicating DNA, detect incorrect bases, excise them, and correctly replace them are ________.

Accepted Answer

A)   DNA ligases
B)   DNA polymerases
C)   DNA helicases
D)   DNA gyrases
E)   primasesF) None of the above
G) C) and D)

Question 20

During protein synthesis, the final mRNA codon on the strand, UAA, recruits which of the following tRNA molecules to the ribosomal complex?

Accepted Answer

A)   tRNA carrying methionine
B)   tRNA carrying proline
C)   tRNA carrying arginine
D)   No corresponding tRNA exists for this codon.E) A) and D)
F) C) and D)

Exam 8: Microbial Genetics and Genetic Engineering

Semiconservative replication refers to ________.

Correct AnswerverifiedShow Answer

The nontranscribed region of DNA to which RNA polymerase binds to initiate transcription is called the ________.

Correct AnswerverifiedShow Answer

The site where the old DNA strands separate and new DNA strands will be synthesized is called the ________.

Correct AnswerverifiedShow Answer

DNA is denatured at ________.

Correct AnswerverifiedShow Answer

A three base sequence on mRNA is called a(n) ________.

Correct AnswerverifiedShow Answer

The RN educates the patient that genetic information can be acquired by bacterial cells in other ways. Which of the following is a correct statement regarding these processes?

Correct AnswerverifiedShow Answer

Three consecutive bases in the DNA of a gene represent the code for one ________.

Correct AnswerverifiedC

Restriction endonucleases recognize and clip at DNA base sequences called ________.

Correct AnswerverifiedShow Answer

RNA molecules differ from DNA molecules because only RNA ________.

Correct AnswerverifiedShow Answer

The smallest unit of heredity is a chromosome.

Correct AnswerverifiedShow Answer

The benefit of the bacterial operon is that ________.

Correct AnswerverifiedShow Answer

Replication of DNA begins at a(n) ________ rich area.

Correct AnswerverifiedShow Answer

Vectors often contain a gene conferring drug resistance to their cloning host, in order to detect cells harboring the plasmid.

Correct AnswerverifiedShow Answer

Which is incorrect about purines?

Correct AnswerverifiedShow Answer

DNA Polymerase I ________.

Correct AnswerverifiedC

Which of the following is not associated with a bacterial ribosome?

Correct AnswerverifiedShow Answer

DNA replication proceeds in one direction around the bacterial chromosome.

Correct AnswerverifiedShow Answer

DNA polymerase III ________.

Correct AnswerverifiedB

The enzymes that can proofread replicating DNA, detect incorrect bases, excise them, and correctly replace them are ________.

Correct AnswerverifiedShow Answer

During protein synthesis, the final mRNA codon on the strand, UAA, recruits which of the following tRNA molecules to the ribosomal complex?

Correct AnswerverifiedShow Answer

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C

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C

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B

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