Question 1

The DNA polymerase III holoenzyme contains two core enzymes,one that is responsible for ___________ strand synthesis and the other responsible for ____________strand synthesis.

Accepted Answer

The answer of The DNA polymerase III holoenzyme contains two...

Question 2

After DNA replication,bacterial genomes must undergo decatenation before the cell can divide.

Accepted Answer

A) True 
 B)False

Question 3

Telomerase enzyme activity was first observed in extracts from Tetrahymena micronuclei.

Accepted Answer

A) True 
 B)False

Question 4

In E.coli,DNA replication begins at a unique site,oriC,in the genome.

Accepted Answer

A) True 
 B)False

Question 5

Which of the following subunits of the DNA polymerase III holoenzyme is referred to as the "sliding clamp"?

Accepted Answer

A)   α\alphaα 
B)   β\betaβ 
C)   γ\gammaγ 
D)   χ\chiχ 
E)   θ	hetaθF) C) and D)
G) A) and B)

Question 6

The DNA polymerase III core plus the ____________ is needed to replicate DNA processively.$\beta$

Accepted Answer

The answer of The DNA polymerase III core plus the...

Question 7

The chromosomal origins of replication vary greatly among bacteria,so much so,that it is hard to propose an origin of replication consensus sequence.

Accepted Answer

A) True 
 B)False

Question 8

Replication of the SV40 genome is dependent on the viral large T antigen,which has primase activity.

Accepted Answer

A) True 
 B)False

Question 9

The eukaryotic processivity factor PCNA serves the same function as the prokaryotic DNA polymerase III $\beta$-subunit.

Accepted Answer

A) True 
 B)False  True

Question 10

All eukaryotes must protect their telomeres from nucleases and double strand break repair enzymes.

Accepted Answer

A) True 
 B)False

Question 11

The ends of eukaryotic chromosomes are composed of short GC-rich repeat sequences called ____________.

Accepted Answer

The answer of The ends of eukaryotic chromosomes are composed...

Question 12

A primosome can be defined as

Accepted Answer

A)  the enzyme that synthesizes primers fro M13 phage replication.
B)  a collection of proteins needed for synthesis of primers for replicating DNA.
C)  a collection of RNA primers.
D)  a DNA molecule that is primed and ready for replication.
E)  an area of DNA near the replication fork waiting to be replicated.F) C) and E)
G) A) and C)

Question 13

Bacterial chromosomes are the only chromosomes known to require decatenation during replication.

Accepted Answer

A) True 
 B)False

Question 14

Telomerase is a unique enzyme in that it is composed of only RNA.

Accepted Answer

A) True 
 B)False

Question 15

In vivo,the DNA polymerase III holoenzyme can synthesize DNA at a rate of

Accepted Answer

A)  250 nt/sec.
B)  500 nt/sec.
C)  750 nt/sec.
D)  1000nt/sec.
E)  2000nt/sec.F) B) and E)
G) C) and D)

Question 16

Without telomeres,linear eukaryotic chromosomes would get shorter and shorter with each round of DNA replication.

Accepted Answer

A) True 
 B)False

Question 17

Which of the following enzymes is responsible for decatenation of Salmonella typhimurium DNA during replication?

Accepted Answer

A)  topoisomerase I
B)  topoisomerase II
C)  topoisomerase III
D)  topoisomerase IV
E)  topoisomerase VF) A) and E)
G) A) and D)

Question 18

During lagging strand synthesis,it is not uncommon for the DNA polymerase III holoenzyme to dissociate completely from the DNA template for approximately two seconds.

Accepted Answer

A) True 
 B)False  False

Question 19

The DNA polymerase III holoenzyme $\gamma$-complex can act as a clamp loader and unloader.

Accepted Answer

A) True 
 B)False

Question 20

DNA polymerase III is a highly ___________ enzyme,meaning once it binds DNA it catalyzes the addition of several hundreds of nucleotides without falling of the DNA.

Accepted Answer

processive

Exam 21: Dna Replication II: Detailed Mechanism

The DNA polymerase III holoenzyme contains two core enzymes,one that is responsible for ___________ strand synthesis and the other responsible for ____________strand synthesis.

Correct AnswerverifiedShow Answer

After DNA replication,bacterial genomes must undergo decatenation before the cell can divide.

Correct AnswerverifiedShow Answer

Telomerase enzyme activity was first observed in extracts from Tetrahymena micronuclei.

Correct AnswerverifiedShow Answer

In E.coli,DNA replication begins at a unique site,oriC,in the genome.

Correct AnswerverifiedShow Answer

Which of the following subunits of the DNA polymerase III holoenzyme is referred to as the "sliding clamp"?

Correct AnswerverifiedShow Answer

The DNA polymerase III core plus the ____________ is needed to replicate DNA processively. β\betaβ

Correct AnswerverifiedShow Answer

The chromosomal origins of replication vary greatly among bacteria,so much so,that it is hard to propose an origin of replication consensus sequence.

Correct AnswerverifiedShow Answer

Replication of the SV40 genome is dependent on the viral large T antigen,which has primase activity.

Correct AnswerverifiedShow Answer

The eukaryotic processivity factor PCNA serves the same function as the prokaryotic DNA polymerase III β\betaβ -subunit.

Correct AnswerverifiedTrue

All eukaryotes must protect their telomeres from nucleases and double strand break repair enzymes.

Correct AnswerverifiedShow Answer

The ends of eukaryotic chromosomes are composed of short GC-rich repeat sequences called ____________.

Correct AnswerverifiedShow Answer

A primosome can be defined as

Correct AnswerverifiedShow Answer

Bacterial chromosomes are the only chromosomes known to require decatenation during replication.

Correct AnswerverifiedShow Answer

Telomerase is a unique enzyme in that it is composed of only RNA.

Correct AnswerverifiedShow Answer

In vivo,the DNA polymerase III holoenzyme can synthesize DNA at a rate of

Correct AnswerverifiedShow Answer

Without telomeres,linear eukaryotic chromosomes would get shorter and shorter with each round of DNA replication.

Correct AnswerverifiedShow Answer

Which of the following enzymes is responsible for decatenation of Salmonella typhimurium DNA during replication?

Correct AnswerverifiedShow Answer

During lagging strand synthesis,it is not uncommon for the DNA polymerase III holoenzyme to dissociate completely from the DNA template for approximately two seconds.

Correct AnswerverifiedFalse

The DNA polymerase III holoenzyme γ\gammaγ -complex can act as a clamp loader and unloader.

Correct AnswerverifiedShow Answer

DNA polymerase III is a highly ___________ enzyme,meaning once it binds DNA it catalyzes the addition of several hundreds of nucleotides without falling of the DNA.

Correct Answerverifiedprocessive

The DNA polymerase III holoenzyme contains two core enzymes,one that is responsible for _ strand synthesis and the other responsible for __strand synthesis.

Correct Answer
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The DNA polymerase III core plus the ____________ is needed to replicate DNA processively. $\beta$

Correct Answer
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Correct Answer
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Correct Answer
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The eukaryotic processivity factor PCNA serves the same function as the prokaryotic DNA polymerase III $\beta$ -subunit.

Correct Answer
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True

Correct Answer
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Correct Answer
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Correct Answer
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Correct Answer
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Correct Answer
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False

The DNA polymerase III holoenzyme $\gamma$ -complex can act as a clamp loader and unloader.

Correct Answer
verified

Correct Answer
verified
processive